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You guys are explaining this so good. I use your video's to show my students about how to troubleshoot!

Thank you for such wonderful explanation.

Nice video! this is in my school materials, i could see what bleeding is on a graph but couldn't put it in the right words. Now i know that it is additional unwanted signals in the graph caused by the degradation of the stationary phase caused by a reactive chemical like a strong acid or base or by oxygen contamination so it is very important to filter for oxygen in the carrier gas.

Hi, thank you for the video! Can you tell me a little bit about the difference between a fixed flow regulator and a variable flow regulator?

Hello, how much is the value of the lamp based on its voltage?

Thanks that was very helpful. Please make a video about maintaining the ECD detector. thanks

After how many injections, (on average), should the filter be replaced? And what about cleaning the split vent lines?

Obrigado

Sou de São Paulo Brasil e trabalho com um cromatógrafo com duas colunas e quando injeto a mesma amostra nos dois, tem muita diferença no resultado, e sinal do fid do front e 6,2 e do back está em 7,8. vou fazer um vídeo e mostrar aqui nos comentários ok.

Obrigado pela informação

Muito obrigado amigo, moro em São Paulo Brasil, e trabalho com um cromatógrafo com duas colunas e uma está com o sinal (fid) muito alto, o front está com 6,2 e o back com 7,8, quando injeto a mesmo amostra nos dois, dá uma diferença, e também no final da corrida e sinal aumenta muito. Vou fazer um vídeo mostrando o ocorrido.

Thank you so much!

The blank must be the same as the mobile phase?

Hi, can you show us how to replace the frit Agilent 1290 system?

do you offer the split vent trap for shimadzu GC2030? The new design for 2030 is offered at hilariously outrageous $200/unit.

You talk about the initial oven temperature, does this mean that the separation was carried out in temperature programming mode? Tank you.

Can you help me, the back detector shows temp not installed. And my infront gas (N2) flow rate i set is 45, but the screen show range 51 - 60. Can you help?

*Make video on TCD*

I have faced an ignition cut-off when the sample was injected. Upto injection of sample flame is on and shows a good signal. but when injected immediately, the ignition will cut. what is the reason? can you please tell me?

I have an Agilent ZORBAX NH2 and Carbohydrate column which is used for HILIC separation for sugar. Since those 2 columns have not been used for a period of time. Which solvents are suitable to use for cleaning and regeneration?

Can you explain what's the purpose of the serrated edge, please? (Assuming there is a purpose).

What is the makeup gas?

Whis is mechanisms of FID detector

How to remove the flowcell contamination?

What is difference between RI,PDA,Uv dectors

How to reduce the column back pressure?

Which solvent suitable for c18 columns?

How to remove the column blockage?

Can one extrapolate two neighbor peaks with resolution below 1.0? Is it correct to integrate the area of extrapolated peaks that are artificially deconvoluted?

Pl.tell about collector assembly sir

What is the principal behind FID Jet

How to clean the FID jet and how to know jet is damaged,?

Good Explanation sir.

What is the minimum syringe injection volume during method optimisation

We are use mostly Exmair syringe.After 100 injection , syringe response wasn't good.What is the best syringe and rugged

What basis syringe(10 micro or 5 micro litre) to be chosen based on injection volume 1 micro litre

Why syringe is struck after 8 injection of DMSO mostly

Thank you for ur guidance ...it's really helpful...thank you...sir

We got solvent peak but no sample peak. Any advice for this? Thanks! Appreciate your assistance.

What about chemicals with labile hydrogen such as carboxylic acid due to the column ? Is there a way to avoid peak tailing ?

Congratulations for the video, but I don't understand how a pulsed split injection can have the liner with increased pressure if the injector outlet opens?

sir why do you need to cut the column? what is the purpose of cutting a column?

Came to watch how to properly re-install the collector assembly. I also am having a hard time.

I am studying for my Chromatography course and this video was literally so well made. Thank you!

Hi sir, In case of benzene in carbomer as per USP method ... the benzene standard getting deterioration after 1 or 2 injections through splitless mode where i have been connected with split line in the GC...is this problem are having connection with split liner...

Could anyone recommend me packed column can separate oxygen, nitrogen and CO to tcd?

Where can I get the cap for the septum purge?

I'm careful if I can ask here, but is it possible to analyze isophthalic acid by GC-MS? I have been having a tailing problem for a month. I will buy all the products you recommend. Somebody help me T^T

Each video is too short,Can some long videos provided to explain the details?